nebnext first strand synthesis kit Search Results


protoscript ii first strand synthesis kit  (New England Biolabs)
96
New England Biolabs protoscript ii first strand synthesis kit
Protoscript Ii First Strand Synthesis Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/protoscript ii first strand synthesis kit/product/New England Biolabs
Average 96 stars, based on 1 article reviews
protoscript ii first strand synthesis kit - by Bioz Stars, 2026-03
96/100 stars
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strand cdna synthesis kit  (TaKaRa)
99
TaKaRa strand cdna synthesis kit
Figure 6. Southern blot analysis of PCR products obtained from <t>total</t> <t>RNA</t> isolated from CD34+ cells transfected with AdCMV-IFN- α gene. Lane 1, PCR products amplified from nontransfected (control) CD34+ cell RNA; lane 2, RNA obtained from CD34+ cells transfected with AdCMV-IFN-α after 24 h; lanes 3 and 4, RNA from CFU-GM clones after 12 and 14 days of culture, respectively; lanes 5 and 6, RNA from BFU-E clones after 12 and 14 days of culture. Total RNA was extracted from CD34+ cells, BFU-E or CFU-GM clones. IFN-α transcripts were amplified by PCR using 2 ml of 100 ml <t>cDNA</t> mixture of a total reaction volume of 25 ml. Aliquots of the reaction mixture (10 ml) were subjected to electrophoresis in a 0.8% agarose (Sigma; St. Louis, MO) gel. Nucleic acids were transferred to nitrocellulose by blotting and probed with IFN-α cDNA probes, (gift of Dr. Asano) using α-32d-CTP. Filters were exposed to x-ray for 48 h at –80°C.
Strand Cdna Synthesis Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/strand cdna synthesis kit/product/TaKaRa
Average 99 stars, based on 1 article reviews
strand cdna synthesis kit - by Bioz Stars, 2026-03
99/100 stars
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first strand cdna synthesis kit  (OriGene)
95
OriGene first strand cdna synthesis kit
Figure 6. Southern blot analysis of PCR products obtained from <t>total</t> <t>RNA</t> isolated from CD34+ cells transfected with AdCMV-IFN- α gene. Lane 1, PCR products amplified from nontransfected (control) CD34+ cell RNA; lane 2, RNA obtained from CD34+ cells transfected with AdCMV-IFN-α after 24 h; lanes 3 and 4, RNA from CFU-GM clones after 12 and 14 days of culture, respectively; lanes 5 and 6, RNA from BFU-E clones after 12 and 14 days of culture. Total RNA was extracted from CD34+ cells, BFU-E or CFU-GM clones. IFN-α transcripts were amplified by PCR using 2 ml of 100 ml <t>cDNA</t> mixture of a total reaction volume of 25 ml. Aliquots of the reaction mixture (10 ml) were subjected to electrophoresis in a 0.8% agarose (Sigma; St. Louis, MO) gel. Nucleic acids were transferred to nitrocellulose by blotting and probed with IFN-α cDNA probes, (gift of Dr. Asano) using α-32d-CTP. Filters were exposed to x-ray for 48 h at –80°C.
First Strand Cdna Synthesis Kit, supplied by OriGene, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/first strand cdna synthesis kit/product/OriGene
Average 95 stars, based on 1 article reviews
first strand cdna synthesis kit - by Bioz Stars, 2026-03
95/100 stars
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first strand cdna synthesis kit  (Pharmacia LKB Biotechnology Inc)
90
Pharmacia LKB Biotechnology Inc first strand cdna synthesis kit
Figure 6. Southern blot analysis of PCR products obtained from <t>total</t> <t>RNA</t> isolated from CD34+ cells transfected with AdCMV-IFN- α gene. Lane 1, PCR products amplified from nontransfected (control) CD34+ cell RNA; lane 2, RNA obtained from CD34+ cells transfected with AdCMV-IFN-α after 24 h; lanes 3 and 4, RNA from CFU-GM clones after 12 and 14 days of culture, respectively; lanes 5 and 6, RNA from BFU-E clones after 12 and 14 days of culture. Total RNA was extracted from CD34+ cells, BFU-E or CFU-GM clones. IFN-α transcripts were amplified by PCR using 2 ml of 100 ml <t>cDNA</t> mixture of a total reaction volume of 25 ml. Aliquots of the reaction mixture (10 ml) were subjected to electrophoresis in a 0.8% agarose (Sigma; St. Louis, MO) gel. Nucleic acids were transferred to nitrocellulose by blotting and probed with IFN-α cDNA probes, (gift of Dr. Asano) using α-32d-CTP. Filters were exposed to x-ray for 48 h at –80°C.
First Strand Cdna Synthesis Kit, supplied by Pharmacia LKB Biotechnology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/first strand cdna synthesis kit/product/Pharmacia LKB Biotechnology Inc
Average 90 stars, based on 1 article reviews
first strand cdna synthesis kit - by Bioz Stars, 2026-03
90/100 stars
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surescript first-strand cdna synthesis kit  (Genecopoeia)
97
Genecopoeia surescript first-strand cdna synthesis kit
Figure 6. Southern blot analysis of PCR products obtained from <t>total</t> <t>RNA</t> isolated from CD34+ cells transfected with AdCMV-IFN- α gene. Lane 1, PCR products amplified from nontransfected (control) CD34+ cell RNA; lane 2, RNA obtained from CD34+ cells transfected with AdCMV-IFN-α after 24 h; lanes 3 and 4, RNA from CFU-GM clones after 12 and 14 days of culture, respectively; lanes 5 and 6, RNA from BFU-E clones after 12 and 14 days of culture. Total RNA was extracted from CD34+ cells, BFU-E or CFU-GM clones. IFN-α transcripts were amplified by PCR using 2 ml of 100 ml <t>cDNA</t> mixture of a total reaction volume of 25 ml. Aliquots of the reaction mixture (10 ml) were subjected to electrophoresis in a 0.8% agarose (Sigma; St. Louis, MO) gel. Nucleic acids were transferred to nitrocellulose by blotting and probed with IFN-α cDNA probes, (gift of Dr. Asano) using α-32d-CTP. Filters were exposed to x-ray for 48 h at –80°C.
Surescript First Strand Cdna Synthesis Kit, supplied by Genecopoeia, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/surescript first-strand cdna synthesis kit/product/Genecopoeia
Average 97 stars, based on 1 article reviews
surescript first-strand cdna synthesis kit - by Bioz Stars, 2026-03
97/100 stars
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protoscript first strand cdna synthesis kit  (New England Biolabs)
96
New England Biolabs protoscript first strand cdna synthesis kit
Figure 6. Southern blot analysis of PCR products obtained from <t>total</t> <t>RNA</t> isolated from CD34+ cells transfected with AdCMV-IFN- α gene. Lane 1, PCR products amplified from nontransfected (control) CD34+ cell RNA; lane 2, RNA obtained from CD34+ cells transfected with AdCMV-IFN-α after 24 h; lanes 3 and 4, RNA from CFU-GM clones after 12 and 14 days of culture, respectively; lanes 5 and 6, RNA from BFU-E clones after 12 and 14 days of culture. Total RNA was extracted from CD34+ cells, BFU-E or CFU-GM clones. IFN-α transcripts were amplified by PCR using 2 ml of 100 ml <t>cDNA</t> mixture of a total reaction volume of 25 ml. Aliquots of the reaction mixture (10 ml) were subjected to electrophoresis in a 0.8% agarose (Sigma; St. Louis, MO) gel. Nucleic acids were transferred to nitrocellulose by blotting and probed with IFN-α cDNA probes, (gift of Dr. Asano) using α-32d-CTP. Filters were exposed to x-ray for 48 h at –80°C.
Protoscript First Strand Cdna Synthesis Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/protoscript first strand cdna synthesis kit/product/New England Biolabs
Average 96 stars, based on 1 article reviews
protoscript first strand cdna synthesis kit - by Bioz Stars, 2026-03
96/100 stars
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protoscript ii first strand cdna synthesis kit  (New England Biolabs)
96
New England Biolabs protoscript ii first strand cdna synthesis kit
Figure 6. Southern blot analysis of PCR products obtained from <t>total</t> <t>RNA</t> isolated from CD34+ cells transfected with AdCMV-IFN- α gene. Lane 1, PCR products amplified from nontransfected (control) CD34+ cell RNA; lane 2, RNA obtained from CD34+ cells transfected with AdCMV-IFN-α after 24 h; lanes 3 and 4, RNA from CFU-GM clones after 12 and 14 days of culture, respectively; lanes 5 and 6, RNA from BFU-E clones after 12 and 14 days of culture. Total RNA was extracted from CD34+ cells, BFU-E or CFU-GM clones. IFN-α transcripts were amplified by PCR using 2 ml of 100 ml <t>cDNA</t> mixture of a total reaction volume of 25 ml. Aliquots of the reaction mixture (10 ml) were subjected to electrophoresis in a 0.8% agarose (Sigma; St. Louis, MO) gel. Nucleic acids were transferred to nitrocellulose by blotting and probed with IFN-α cDNA probes, (gift of Dr. Asano) using α-32d-CTP. Filters were exposed to x-ray for 48 h at –80°C.
Protoscript Ii First Strand Cdna Synthesis Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/protoscript ii first strand cdna synthesis kit/product/New England Biolabs
Average 96 stars, based on 1 article reviews
protoscript ii first strand cdna synthesis kit - by Bioz Stars, 2026-03
96/100 stars
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first strand synthesis kit  (Quanta Biosciences)
97
Quanta Biosciences first strand synthesis kit
Figure 6. Southern blot analysis of PCR products obtained from <t>total</t> <t>RNA</t> isolated from CD34+ cells transfected with AdCMV-IFN- α gene. Lane 1, PCR products amplified from nontransfected (control) CD34+ cell RNA; lane 2, RNA obtained from CD34+ cells transfected with AdCMV-IFN-α after 24 h; lanes 3 and 4, RNA from CFU-GM clones after 12 and 14 days of culture, respectively; lanes 5 and 6, RNA from BFU-E clones after 12 and 14 days of culture. Total RNA was extracted from CD34+ cells, BFU-E or CFU-GM clones. IFN-α transcripts were amplified by PCR using 2 ml of 100 ml <t>cDNA</t> mixture of a total reaction volume of 25 ml. Aliquots of the reaction mixture (10 ml) were subjected to electrophoresis in a 0.8% agarose (Sigma; St. Louis, MO) gel. Nucleic acids were transferred to nitrocellulose by blotting and probed with IFN-α cDNA probes, (gift of Dr. Asano) using α-32d-CTP. Filters were exposed to x-ray for 48 h at –80°C.
First Strand Synthesis Kit, supplied by Quanta Biosciences, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/first strand synthesis kit/product/Quanta Biosciences
Average 97 stars, based on 1 article reviews
first strand synthesis kit - by Bioz Stars, 2026-03
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reverse transcriptase pcr (rt-pcr) kit  (Agilent technologies)
90
Agilent technologies reverse transcriptase pcr (rt-pcr) kit
Figure 6. Southern blot analysis of PCR products obtained from <t>total</t> <t>RNA</t> isolated from CD34+ cells transfected with AdCMV-IFN- α gene. Lane 1, PCR products amplified from nontransfected (control) CD34+ cell RNA; lane 2, RNA obtained from CD34+ cells transfected with AdCMV-IFN-α after 24 h; lanes 3 and 4, RNA from CFU-GM clones after 12 and 14 days of culture, respectively; lanes 5 and 6, RNA from BFU-E clones after 12 and 14 days of culture. Total RNA was extracted from CD34+ cells, BFU-E or CFU-GM clones. IFN-α transcripts were amplified by PCR using 2 ml of 100 ml <t>cDNA</t> mixture of a total reaction volume of 25 ml. Aliquots of the reaction mixture (10 ml) were subjected to electrophoresis in a 0.8% agarose (Sigma; St. Louis, MO) gel. Nucleic acids were transferred to nitrocellulose by blotting and probed with IFN-α cDNA probes, (gift of Dr. Asano) using α-32d-CTP. Filters were exposed to x-ray for 48 h at –80°C.
Reverse Transcriptase Pcr (Rt Pcr) Kit, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/reverse transcriptase pcr (rt-pcr) kit/product/Agilent technologies
Average 90 stars, based on 1 article reviews
reverse transcriptase pcr (rt-pcr) kit - by Bioz Stars, 2026-03
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primescript rt reagent kit  (TaKaRa)
99
TaKaRa primescript rt reagent kit
Figure 6. Southern blot analysis of PCR products obtained from <t>total</t> <t>RNA</t> isolated from CD34+ cells transfected with AdCMV-IFN- α gene. Lane 1, PCR products amplified from nontransfected (control) CD34+ cell RNA; lane 2, RNA obtained from CD34+ cells transfected with AdCMV-IFN-α after 24 h; lanes 3 and 4, RNA from CFU-GM clones after 12 and 14 days of culture, respectively; lanes 5 and 6, RNA from BFU-E clones after 12 and 14 days of culture. Total RNA was extracted from CD34+ cells, BFU-E or CFU-GM clones. IFN-α transcripts were amplified by PCR using 2 ml of 100 ml <t>cDNA</t> mixture of a total reaction volume of 25 ml. Aliquots of the reaction mixture (10 ml) were subjected to electrophoresis in a 0.8% agarose (Sigma; St. Louis, MO) gel. Nucleic acids were transferred to nitrocellulose by blotting and probed with IFN-α cDNA probes, (gift of Dr. Asano) using α-32d-CTP. Filters were exposed to x-ray for 48 h at –80°C.
Primescript Rt Reagent Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/primescript rt reagent kit/product/TaKaRa
Average 99 stars, based on 1 article reviews
primescript rt reagent kit - by Bioz Stars, 2026-03
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rna revertaid first strand cdna synthesis kit  (Thermo Fisher)
90
Thermo Fisher rna revertaid first strand cdna synthesis kit
Figure 6. Southern blot analysis of PCR products obtained from <t>total</t> <t>RNA</t> isolated from CD34+ cells transfected with AdCMV-IFN- α gene. Lane 1, PCR products amplified from nontransfected (control) CD34+ cell RNA; lane 2, RNA obtained from CD34+ cells transfected with AdCMV-IFN-α after 24 h; lanes 3 and 4, RNA from CFU-GM clones after 12 and 14 days of culture, respectively; lanes 5 and 6, RNA from BFU-E clones after 12 and 14 days of culture. Total RNA was extracted from CD34+ cells, BFU-E or CFU-GM clones. IFN-α transcripts were amplified by PCR using 2 ml of 100 ml <t>cDNA</t> mixture of a total reaction volume of 25 ml. Aliquots of the reaction mixture (10 ml) were subjected to electrophoresis in a 0.8% agarose (Sigma; St. Louis, MO) gel. Nucleic acids were transferred to nitrocellulose by blotting and probed with IFN-α cDNA probes, (gift of Dr. Asano) using α-32d-CTP. Filters were exposed to x-ray for 48 h at –80°C.
Rna Revertaid First Strand Cdna Synthesis Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rna revertaid first strand cdna synthesis kit/product/Thermo Fisher
Average 90 stars, based on 1 article reviews
rna revertaid first strand cdna synthesis kit - by Bioz Stars, 2026-03
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transcriptor first strand cdna synthesis kit  (Roche)
86
Roche transcriptor first strand cdna synthesis kit
Figure 6. Southern blot analysis of PCR products obtained from <t>total</t> <t>RNA</t> isolated from CD34+ cells transfected with AdCMV-IFN- α gene. Lane 1, PCR products amplified from nontransfected (control) CD34+ cell RNA; lane 2, RNA obtained from CD34+ cells transfected with AdCMV-IFN-α after 24 h; lanes 3 and 4, RNA from CFU-GM clones after 12 and 14 days of culture, respectively; lanes 5 and 6, RNA from BFU-E clones after 12 and 14 days of culture. Total RNA was extracted from CD34+ cells, BFU-E or CFU-GM clones. IFN-α transcripts were amplified by PCR using 2 ml of 100 ml <t>cDNA</t> mixture of a total reaction volume of 25 ml. Aliquots of the reaction mixture (10 ml) were subjected to electrophoresis in a 0.8% agarose (Sigma; St. Louis, MO) gel. Nucleic acids were transferred to nitrocellulose by blotting and probed with IFN-α cDNA probes, (gift of Dr. Asano) using α-32d-CTP. Filters were exposed to x-ray for 48 h at –80°C.
Transcriptor First Strand Cdna Synthesis Kit, supplied by Roche, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/transcriptor first strand cdna synthesis kit/product/Roche
Average 86 stars, based on 1 article reviews
transcriptor first strand cdna synthesis kit - by Bioz Stars, 2026-03
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Figure 6. Southern blot analysis of PCR products obtained from total RNA isolated from CD34+ cells transfected with AdCMV-IFN- α gene. Lane 1, PCR products amplified from nontransfected (control) CD34+ cell RNA; lane 2, RNA obtained from CD34+ cells transfected with AdCMV-IFN-α after 24 h; lanes 3 and 4, RNA from CFU-GM clones after 12 and 14 days of culture, respectively; lanes 5 and 6, RNA from BFU-E clones after 12 and 14 days of culture. Total RNA was extracted from CD34+ cells, BFU-E or CFU-GM clones. IFN-α transcripts were amplified by PCR using 2 ml of 100 ml cDNA mixture of a total reaction volume of 25 ml. Aliquots of the reaction mixture (10 ml) were subjected to electrophoresis in a 0.8% agarose (Sigma; St. Louis, MO) gel. Nucleic acids were transferred to nitrocellulose by blotting and probed with IFN-α cDNA probes, (gift of Dr. Asano) using α-32d-CTP. Filters were exposed to x-ray for 48 h at –80°C.

Journal: Stem cells (Dayton, Ohio)

Article Title: Adenovirus mediated alpha interferon (IFN-alpha) gene transfer into CD34+ cells and CML mononuclear cells.

doi: 10.1002/stem.150386

Figure Lengend Snippet: Figure 6. Southern blot analysis of PCR products obtained from total RNA isolated from CD34+ cells transfected with AdCMV-IFN- α gene. Lane 1, PCR products amplified from nontransfected (control) CD34+ cell RNA; lane 2, RNA obtained from CD34+ cells transfected with AdCMV-IFN-α after 24 h; lanes 3 and 4, RNA from CFU-GM clones after 12 and 14 days of culture, respectively; lanes 5 and 6, RNA from BFU-E clones after 12 and 14 days of culture. Total RNA was extracted from CD34+ cells, BFU-E or CFU-GM clones. IFN-α transcripts were amplified by PCR using 2 ml of 100 ml cDNA mixture of a total reaction volume of 25 ml. Aliquots of the reaction mixture (10 ml) were subjected to electrophoresis in a 0.8% agarose (Sigma; St. Louis, MO) gel. Nucleic acids were transferred to nitrocellulose by blotting and probed with IFN-α cDNA probes, (gift of Dr. Asano) using α-32d-CTP. Filters were exposed to x-ray for 48 h at –80°C.

Article Snippet: RNA is reverse-transcribed using the first strand cDNA synthesis kit from Clontech, Inc. (Palo Alto, CA).

Techniques: Southern Blot, Isolation, Transfection, Amplification, Control, Clone Assay, Electrophoresis

Figure 7. Southern blot analysis of PCR products obtained from total RNA isolated from CML hematopoietic stem cells (BMMNC) transfected with AdCMV-IFN-α gene. Lane 1, PCR products amplified from nontransfected (control) cell RNA; lane 2, RNA obtained from cells transfected with AdCMV-IFN-α after 24 h; lane 3, RNA from CFU-GM clones after 12 days; lane 4, CFU-GM clones after 14 days of culture; lane 5, no RNA was added; and lane 6, positive control. Total RNA was extracted from stem cells or CFU-GM clones by one single extraction. cDNA was synthesized from total RNA. IFN-α transcripts were amplified by PCR using 2 ml of 100 ml cDNA mixture of a total reaction vol- ume of 25 ml. Aliquots of the reaction mixture (10 ml) were sub- jected to electrophoresis in a 0.8% agarose (Sigma) gel. Nucleic acids were transferred to nitrocellulose by blotting and probed with IFN-α cDNA probes, using α-32P-CTP. Filters were exposed to x-ray for 48 h at –80°C.

Journal: Stem cells (Dayton, Ohio)

Article Title: Adenovirus mediated alpha interferon (IFN-alpha) gene transfer into CD34+ cells and CML mononuclear cells.

doi: 10.1002/stem.150386

Figure Lengend Snippet: Figure 7. Southern blot analysis of PCR products obtained from total RNA isolated from CML hematopoietic stem cells (BMMNC) transfected with AdCMV-IFN-α gene. Lane 1, PCR products amplified from nontransfected (control) cell RNA; lane 2, RNA obtained from cells transfected with AdCMV-IFN-α after 24 h; lane 3, RNA from CFU-GM clones after 12 days; lane 4, CFU-GM clones after 14 days of culture; lane 5, no RNA was added; and lane 6, positive control. Total RNA was extracted from stem cells or CFU-GM clones by one single extraction. cDNA was synthesized from total RNA. IFN-α transcripts were amplified by PCR using 2 ml of 100 ml cDNA mixture of a total reaction vol- ume of 25 ml. Aliquots of the reaction mixture (10 ml) were sub- jected to electrophoresis in a 0.8% agarose (Sigma) gel. Nucleic acids were transferred to nitrocellulose by blotting and probed with IFN-α cDNA probes, using α-32P-CTP. Filters were exposed to x-ray for 48 h at –80°C.

Article Snippet: RNA is reverse-transcribed using the first strand cDNA synthesis kit from Clontech, Inc. (Palo Alto, CA).

Techniques: Southern Blot, Isolation, Transfection, Amplification, Control, Clone Assay, Positive Control, Extraction, Synthesized, Electrophoresis